PCR detection of theTth111 I RFLP at the RB locus
نویسندگان
چکیده
منابع مشابه
Asp700 RFLP at the D8S45 locus.
Source/Description of Clone: Clone pALP13-8 is a single copy 750 bp EcoRV fragment, 0.9 kb 3' of exon 4, subcloned from a 6.6 kb BamHI fragment containing the complete human c-fos oncogene (FOS) (1). Polymorphisms: are detected with TaqI (A alleles) and BstNI (B alleles) (see Figure). A) TaqI: Presence of a 1.6 kb fragment or its allele, a 0.6 kb fragment. B) BstNI: Presence of an 0.85 kb fragm...
متن کاملPCR detection of the TAQ1 polymorphism at the CA2 locus.
In humans, at least seven isozymes of carbonic anhydrase (CA1—CA7) are known, five of which are encoded by genes on chromosomes 1 (CA6), 8 (CA1-3) and 16 (CAT) (1). We have previously described a Taql RFLP with a 50% allele frequency near the CA2 gene (2). We have now bracketed the site with PCR (3) primers to develop an STS site. The original DNA samples (2) produced the same typings (+ = Taql...
متن کاملAva I RFLP at the gas-1 locus on mouse chromosome 12.
SOURCE/DESCRIPTION A family of G-specific genes vhose expression is negatively regulated by serum and growth factor has been called gas (growth-arrest-spp.cif lc genes). A fragment of 1.3 Kb of gas-1 cDNA cloned in a EcoRI site of Blue script vector vas used as probe. POLYMORPHISM After DNA digestion with Ava I a constant band of 4.9 Kb and a variant band of 5.4 Kb were observed. FREQUENCY The ...
متن کاملA BstE II RFLP at the DXS153 locus.
Source/Description: cpX12 is a 1.1 kb EcoRI single-copy genomic fragment cloned into pAT153 from a pool of 100 cosmids isolated from a human/hamster somatic cell hybrid containing only the human X chromosome (1). Polymorphism: cpX12 reveals a two-allele restriction fragment length polymorphism with bands at 0.7 kb and 1.9 kb (Al) or 2.6 kb (A2) after Southern blot analysis of human genomic DNA ...
متن کاملRapid communication: A PCR-RFLP marker at the porcine complement factor B gene locus shows between-population frequency variation.
1998 American Society of Animal Science. All rights reserved. Figure 1. PCR-RFLP at porcine BF locus. Lane 1: PCR product, 390 bp. Lane 2: 100 bp ladder (New England Biolabs, Beverly, MA). Lanes 3, 4 and 7: homozygotes SmaI+/SmaI+, 237+153 bp. Lanes 5 and 6: heterozygotes SmaI+/SmaI−, 390+237+153 bp. Lanes 8 and 9: homozygotes SmaI−/SmaI−, 390 bp. Rapid Communication: A PCR-RFLP Marker at the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.16.4965